Performing Analysis

Starting a Run

If Mass Calibration / Check was performed the LC flow will have been set to go to the MS automatically. Otherwise, perform the following steps to set the flow:

  1. Right click on the QTOF panel of the Instrument Status window in MassHunter

  2. Select LC → Waste from the menu.

    The Instrument Status window

    The Instrument Status window

To start the run, in the Worklist, select the samples to run by ticking the box to the left of the Sample Name field and click the ▶ button.

View of the Worklist

The Worklist

During the Run

  1. Check that the Column pressure remains stable and does not rise above 200 bar. Analysis should stop automatically if the pressure rises above 600 bar.
  2. Check that the three symbols in the Q-TOF panel of the Instrument Status window (shown below) remain green to indicate that the reference ions were found.
The Instrument Status window

The Instrument Status window

Shutdown

  1. In the Instrument Status window, click the Off button to the lower right.
  2. Close Data Acquisition.